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| Registros recuperados: 13 | |
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| Carradec, Quentin; Pelletier, Eric; Da Silva, Corinne; Alberti, Adriana; Seeleuthner, Yoann; Blanc-mathieu, Romain; Lima-mendez, Gipsi; Rocha, Fabio; Tirichine, Leila; Labadie, Karine; Kirilovsky, Amos; Bertrand, Alexis; Engelen, Stefan; Madoui, Mohammed-amin; Meheust, Raphael; Poulain, Julie; Romac, Sarah; Richter, Daniel J.; Yoshikawa, Genki; Dimier, Celine; Kandels-lewis, Stefanie; Picheral, Marc; Searson, Sarah; Jaillon, Olivier; Aury, Jean-marc; Karsenti, Eric; Sullivan, Matthew B.; Sunagawa, Shinichi; Bork, Peer; Not, Fabrice; Hingamp, Pascal; Raes, Jeroen; Guidi, Lionel; Ogata, Hiroyuki; De Vargas, Colomban; Iudicone, Daniele; Bowler, Chris; Wincker, Patrick; Tara Oceans Coordinators,. |
| While our knowledge about the roles of microbes and viruses in the ocean has increased tremendously due to recent advances in genomics and metagenomics, research on marine microbial eukaryotes and zooplankton has benefited much less from these new technologies because of their larger genomes, their enormous diversity, and largely unexplored physiologies. Here, we use a metatranscriptomics approach to capture expressed genes in open ocean Tara Oceans stations across four organismal size fractions. The individual sequence reads cluster into 116 million unigenes representing the largest reference collection of eukaryotic transcripts from any single biome. The catalog is used to unveil functions expressed by eukaryotic marine plankton, and to assess their... |
| Tipo: Text |
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| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00660/77232/79053.pdf |
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| Nanjappa, Deepak; Audic, Stephane; Romac, Sarah; Kooistra, Wiebe H. C. F.; Zingone, Adriana. |
| Background: Continuous efforts to estimate actual diversity and to trace the species distribution and ranges in the natural environments have gone in equal pace with advancements of the technologies in the study of microbial species diversity from microscopic observations to DNA-based barcoding. DNA metabarcoding based on Next Generation Sequencing (NGS) constitutes the latest advancement in these efforts. Here we use NGS data from different sites to investigate the geographic range of six species of the diatom family Leptocylindraceae and to identify possible new taxa within the family. Methodology/Principal Findings: We analysed the V4 and V9 regions of the nuclear-encoded SSU rDNA gene region in the NGS database of the European ERA-Biodiversa project... |
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| Ano: 2014 |
URL: https://archimer.ifremer.fr/doc/00374/48494/48846.pdf |
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| Forster, Dominik; Dunthorn, Micah; Mahe, Frederic; Dolan, John R.; Audic, Stephane; Bass, David; Bittner, Lucie; Boutte, Christophe; Christen, Richard; Claverie, Jean-michel; Decelle, Johan; Edvardsen, Bente; Egge, Elianne; Eikrem, Wenche; Gobet, Angelique; Kooistra, Wiebe H. C. F.; Logares, Ramiro; Massana, Ramon; Montresor, Marina; Not, Fabrice; Ogata, Hiroyuki; Pawlowski, Jan; Pernice, Massimo C.; Romac, Sarah; Shalchian-tabrizi, Kamran; Simon, Nathalie; Richards, Thomas A.; Santini, Sebastien; Sarno, Diana; Siano, Raffaele; Vaulot, Daniel; Wincker, Patrick; Zingone, Adriana; De Vargas, Colomban; Stoeck, Thorsten. |
| Marine protist diversity inventories have largely focused on planktonic environments, while benthic protists have received relatively little attention. We therefore hypothesize that current diversity surveys have only skimmed the surface of protist diversity in marine sediments, which may harbour greater diversity than planktonic environments. We tested this by analyzing sequences of the hypervariable V4 18S rRNA from benthic and planktonic protist communities sampled in European coastal regions. Despite a similar number of OTUs in both realms, richness estimations indicated that we recovered at least 70% of the diversity in planktonic protist communities, but only 33% in benthic communities. There was also little overlap of OTUs between planktonic and... |
| Tipo: Text |
Palavras-chave: Benthic microbial communities; Coastal environments; Protist diversity; High-throughput sequencing. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00341/45233/44658.pdf |
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| Ramond, Pierre; Sourisseau, Marc; Simon, Nathalie; Romac, Sarah; Schmitt, Sophie; Rigaut-jalabert, Fabienne; Henry, Nicolas; De Vargas, Colomban; Siano, Raffaele. |
| The study of protistan functional diversity is crucial to understand the dynamics of oceanic ecological processes. We combined the metabarcoding data of various coastal ecosystems and a newly developed trait‐based approach to study the link between taxonomic and functional diversity across marine protistan communities of different size‐classes. Environmental DNA was extracted and the V4 18S rDNA genomic region was amplified and sequenced. In parallel, we tried to annotate the Operational Taxonomic Units (OTUs) from our metabarcoding dataset to 30 biological traits using published and accessible information on protists. We then developed a method to study trait correlations across protists (i.e. trade‐offs) in order to build the best functional groups.... |
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| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00478/58964/61554.pdf |
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| De Vargas, Colomban; Audic, Stephane; Henry, Nicolas; Decelle, Johan; Mahe, Frederic; Logares, Ramiro; Lara, Enrique; Berney, Cedric; Le Bescot, Noan; Probert, Ian; Carmichael, Margaux; Poulain, Julie; Romac, Sarah; Colin, Sebastien; Aury, Jean-marc; Bittner, Lucie; Chaffron, Samuel; Dunthorn, Micah; Engelen, Stefan; Flegontova, Olga; Guidi, Lionel; Horak, Ales; Jaillon, Olivier; Lima-mendez, Gipsi; Lukes, Julius; Malviya, Shruti; Morard, Raphael; Mulot, Matthieu; Scalco, Eleonora; Siano, Raffaele; Vincent, Flora; Zingone, Adriana; Dimier, Celine; Picheral, Marc; Searson, Sarah; Kandels-lewis, Stefanie; Acinas, Silvia G.; Bork, Peer; Bowler, Chris; Gorsky, Gabriel; Grimsley, Nigel; Hingamp, Pascal; Iudicone, Daniele; Not, Fabrice; Ogata, Hiroyuki; Pesant, Stephane; Raes, Jeroen; Sieracki, Michael E.; Speich, Sabrina; Stemmann, Lars; Sunagawa, Shinichi; Weissenbach, Jean; Wincker, Patrick; Karsenti, Eric. |
| Marine plankton support global biological and geochemical processes. Surveys of their biodiversity have hitherto been geographically restricted and have not accounted for the full range of plankton size. We assessed eukaryotic diversity from 334 size-fractionated photic-zone plankton communities collected across tropical and temperate oceans during the circumglobal Tara Oceans expedition. We analyzed 18S ribosomal DNA sequences across the intermediate plankton-size spectrum from the smallest unicellular eukaryotes (protists, > 0.8 micrometers) to small animals of a few millimeters. Eukaryotic ribosomal diversity saturated at similar to 150,000 operational taxonomic units, about one-third of which could not be assigned to known eukaryotic groups.... |
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| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00270/38135/37217.pdf |
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| Gorsky, Gabriel; Bourdin, Guillaume; Lombard, Fabien; Pedrotti, Maria Luiza; Audrain, Samuel; Bin, Nicolas; Boss, Emmanuel; Bowler, Chris; Cassar, Nicolas; Caudan, Loic; Chabot, Genevieve; Cohen, Natalie R.; Cron, Daniel; De Vargas, Colomban; Dolan, John R.; Douville, Eric; Elineau, Amanda; Flores, J. Michel; Ghiglione, Jean Francois; Haentjens, Nils; Hertau, Martin; John, Seth G.; Kelly, Rachel L.; Koren, Ilan; Lin, Yajuan; Marie, Dominique; Moulin, Clementine; Moucherie, Yohann; Pesant, Stephane; Picheral, Marc; Poulain, Julie; Pujo-pay, Mireille; Reverdin, Gilles; Romac, Sarah; Sullivan, Mathew B.; Trainic, Miri; Tressol, Marc; Trouble, Romain; Vardi, Assaf; Voolstra, Christian R.; Wincker, Patrick; Agostini, Sylvain; Banaigs, Bernard; Boissin, Emilie; Forcioli, Didier; Furla, Paola; Galand, Pierre E.; Gilson, Eric; Reynaud, Stephanie; Sunagawa, Shinichi; Thomas, Olivier P.; Thurber, Rebecca Lisette Vega; Zoccola, Didier; Planes, Serge; Allemand, Denis; Karsenti, Eric; Planes, S.; Banaig, B.; Boissin, E.; Iwankow, G.; Allemand, D.; Zoccola, D.; Reynaud, S.; Beraud, E.; Djerbi, N.; Forcioli, D.; Furla, P.; Gilson, E.; Mcmind, R.; Ottaviani, A.; Rottinger, E.; Rouan, A.; Zamoum, T.; Flume, B. C. C.; Pogoreutz, C.; Voolstra, C. R.; Rothig, T.; Ziegler, M.; Paoli, L.; Ruscheweyh, H-j; Salazar, G.; Sunagawa, S.; Flores, J. M.; Koren, I; Trainic, M.; Lang-yona, N.; Vardi, A.; Conan, P.; Ghiglione, J-f; Pujo-pay, M.; Galand, P. E.; Hochart, C.; Audrain, S.; Bourgois, E.; Hertau, M.; Lancelot, J.; Monmarche, D.; Moulin, C.; Moucherie, Y.; Trouble, R.; Boss, E.; Bourdin, G.; Haentjens, N.; Karp-boss, L.; Douville, Eric; Agostini, S.; Mitsuhashi, G.; Kitano, Y.; Da Silva, O.; Dolan, J. R.; Gorsky, G.; Lemee, R.; Lombard, F.; Pedrotti, M-l; Cronin, D.; Sullivan, M.; Armstrong, E.; Aury, J-m; Barbe, V; Belser, C.; Carradec, Q.; Labadie, K.; Le-hoang, J.; Noel, B.; Poulain, J.; Wincker, P.; Klinges, G.; Vega-thunder, R.; Bonnival, E.; De Vargas, C.; Henry, N.; Marie, D.; Romac, S.; Pesant, S.; Miguel-gorda, M.; Thomas, O. P.; Bowler, C.; Friedrich, R.; Cassar, N.; Lin, Y.; John, S. G.; Kelly, R. L.; Cohen, N. R.; Reverdin, G.; Filee, J.. |
| Interactions between the ocean and the atmosphere occur at the air-sea interface through the transfer of momentum, heat, gases and particulate matter, and through the impact of the upper-ocean biology on the composition and radiative properties of this boundary layer. The Tara Pacific expedition, launched in May 2016 aboard the schooner Tara, was a 29-month exploration with the dual goals to study the ecology of reef ecosystems along ecological gradients in the Pacific Ocean and to assess inter-island and open ocean surface plankton and neuston community structures. In addition, key atmospheric properties were measured to study links between the two boundary layer properties. A major challenge for the open ocean sampling was the lack of ship-time available... |
| Tipo: Text |
Palavras-chave: Neuston/plankton genomics/taxonomy/imaging; Aerosols; NCP; IOP; Trace metals; Microplastic. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00599/71083/69390.pdf |
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| Rodriguez-martinez, Raquel; Rocap, Gabrielle; Logares, Ramiro; Romac, Sarah; Massana, Ramon. |
| Recent culture-independent studies of marine planktonic protists have unveiled a large diversity at all phylogenetic scales and the existence of novel groups. MAST-4 represents one of these novel uncultured lineages, and it is composed of small (similar to 2 mu m) bacterivorous eukaryotes that are widely distributed in marine systems. MAST-4 accounts for a significant fraction of the marine heterotrophic flagellates at the global level, playing key roles in the marine ecological network. In this study, we investigated the diversity of MAST-4, aiming to assess its limits and structure. Using ribosomal DNA (rDNA) sequences obtained in this study (both pyrosequencing reads and clones with large rDNA operon coverage), complemented with GenBank sequences, we... |
| Tipo: Text |
Palavras-chave: MAST-4; Low evolutionary diversification; Uncultured protist; Pyrosequencing; ITS secondary structure. |
| Ano: 2012 |
URL: https://archimer.ifremer.fr/doc/00374/48506/48778.pdf |
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| Massana, Ramon; Gobet, Angelique; Audic, Stephane; Bass, David; Bittner, Lucie; Boutte, Christophe; Chambouvet, Aurelie; Christen, Richard; Claverie, Jean-michel; Decelle, Johan; Dolan, John R.; Dunthorn, Micah; Edvardsen, Bente; Forn, Irene; Forster, Dominik; Guillou, Laure; Jaillon, Olivier; Kooistra, Wiebe H. C. F.; Logares, Ramiro; Mahe, Frederic; Not, Fabrice; Ogata, Hiroyuki; Pawlowski, Jan; Pernice, Massimo C.; Probert, Ian; Romac, Sarah; Richards, Thomas; Santini, Sebastien; Shalchian-tabrizi, Kamran; Siano, Raffaele; Simon, Nathalie; Stoeck, Thorsten; Vaulot, Daniel; Zingone, Adriana; De Vargas, Colomban. |
| Although protists are critical components of marine ecosystems, they are still poorly characterized. Here we analysed the taxonomic diversity of planktonic and benthic protist communities collected in six distant European coastal sites. Environmental deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) from three size fractions (pico-, nano- and micro/mesoplankton), as well as from dissolved DNA and surface sediments were used as templates for tag pyrosequencing of the V4 region of the 18S ribosomal DNA. Beta-diversity analyses split the protist community structure into three main clusters: picoplankton-nanoplankton-dissolved DNA, micro/mesoplankton and sediments. Within each cluster, protist communities from the same site and time clustered together,... |
| Tipo: Text |
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| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00275/38594/37190.pdf |
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| Richards, Thomas A.; Leonard, Guy; Mahe, Frederic; Del Campov, Javier; Romac, Sarah; Jones, Meredith D. M.; Maguire, Finlay; Dunthorn, Micah; De Vargas, Colomban; Massana, Ramon; Chambouvet, Aurelie. |
| Environmental DNA and culture-based analyses have suggested that fungi are present in low diversity and in low abundance in many marine environments, especially in the upper water column. Here, we use a dual approach involving high-throughput diversity tag sequencing from both DNA and RNA templates and fluorescent cell counts to evaluate the diversity and relative abundance of fungi across marine samples taken from six European near-shore sites. We removed very rare fungal operational taxonomic units (OTUs) selecting only OTUs recovered from multiple samples for a detailed analysis. This approach identified a set of 71 fungal 'OTU clusters' that account for 66% of all the sequences assigned to the Fungi. Phylogenetic analyses demonstrated that this... |
| Tipo: Text |
Palavras-chave: 454 pyrosequencing; Chytrids; Dikarya; Sediment communities. |
| Ano: 2015 |
URL: https://archimer.ifremer.fr/doc/00374/48490/48880.pdf |
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| Logares, Ramiro; Audic, Stephane; Bass, David; Bittner, Lucie; Boutte, Christophe; Christen, Richard; Claverie, Jean-michel; Decelle, Johan; Dolan, John R.; Dunthorn, Micah; Edvardsen, Bente; Gobet, Angelique; Kooistra, Wiebe H. C. F.; Mahe, Frederic; Not, Fabrice; Ogata, Hiroyuki; Pawlowski, Jan; Pernice, Massimo C.; Romac, Sarah; Shalchian-tabrizi, Kamran; Simon, Nathalie; Stoeck, Thorsten; Santini, Sebastien; Siano, Raffaele; Wincker, Patrick; Zingone, Adriana; Richards, Thomas A.; De Vargas, Colomban; Massana, Ramon. |
| Background Biological communities are normally composed of a few abundant and many rare species. This pattern is particularly prominent in microbial communities, in which most constituent taxa are usually extremely rare. Although abundant and rare subcommunities may present intrinsic characteristics that could be crucial for understanding community dynamics and ecosystem functioning, microbiologists normally do not differentiate between them. Here, we investigate abundant and rare subcommunities of marine microbial eukaryotes, a crucial group of organisms that remains among the least-explored biodiversity components of the biosphere. We surveyed surface waters of six separate coastal locations in Europe, independently considering the picoplankton,... |
| Tipo: Text |
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| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00186/29683/28098.pdf |
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| Planes, Serge; Allemand, Denis; Agostini, Sylvain; Banaigs, Bernard; Boissin, Emilie; Boss, Emmanuel; Bourdin, Guillaume; Bowler, Chris; Douville, Eric; Flores, J. Michel; Forcioli, Didier; Furla, Paola; Galand, Pierre E.; Ghiglione, Jean-francois; Gilson, Eric; Lombard, Fabien; Moulin, Clementine; Pesant, Stephane; Poulain, Julie; Reynaud, Stephanie; Romac, Sarah; Sullivan, Matthew B.; Sunagawa, Shinichi; Thomas, Olivier P.; Trouble, Romain; De Vargas, Colomban; Thurber, Rebecca Vega; Voolstra, Christian R.; Wincker, Patrick; Zoccola, Didier; Planes, S.; Allemand, D.; Agostini, S.; Armstrong, E.; Audrain, S.; Aury, J-m; Banaig, B.; Barbe, V; Belser, C.; Beraud, E.; Boissin, E.; Bonnival, E.; Boss, E.; Bourdin, G.; Bourgois, E.; Bowler, C.; Carradec, Q.; Cassar, N.; Cohen, N. R.; Conan, P.; Cronin, D. R.; Da Silva, O.; De Vargas, C.; Djerbi, N.; Dolan, J. R.; Herta, Dominguez G.; Douville, Eric; Du J,; Filee, J.; Flores, J. M.; Forcioli, D.; Friedrich, R.; Furla, P.; Galand, P. E.; Ghiglione, J-f; Gilson, E.; Gorsky, G.; Guinther, M.; Haentjens, N.; Henry, N.; Hertau, M.; Hochart, C.; Hume, B. C. C.; Iwankow, G.; John, S. G.; Karp-boss, L.; Kelly, R. L.; Kitano, Y.; Klinges, G.; Koren, I; Labadie, K.; Lancelot, J.; Lang-yona, N.; Le-hoang, J.; Lemee, R.; Lin, Y.; Lombard, F.; Marie, D.; Mcmind, R.; Miguel-gordo, M.; Trainic, M.; Monmarche, D.; Moulin, C.; Mucherie, Y.; Noel, B.; Ottaviani, A.; Paoli, L.; Pedrotti, M-l; Pesant, S.; Pogoreutz, C.; Poulain, J.; Pujo-pay, M.; Reverdin, G.; Reynaud, S.; Romac, S.; Rothig, T.; Rottinger, E.; Rouan, A.; Ruscheweyh, H-j; Salazar, G.; Sullivan, M. B.; Sunagawa, S.; Thomas, O. P.; Trouble, R.; Vardi, A.; Vega-thunder, R.; Voolstra, C. R.; Wincker, P.; Zahed, A.; Zamoum, T.; Ziegler, M.; Zoccola, D.. |
| Coral reefs are the most diverse habitats in the marine realm. Their productivity, structural complexity, and biodiversity critically depend on ecosystem services provided by corals that are threatened because of climate change effects-in particular, ocean warming and acidification. The coral holobiont is composed of the coral animal host, endosymbiotic dinoflagellates, associated viruses, bacteria, and other microeukaryotes. In particular, the mandatory photosymbiosis with microalgae of the family Symbiodiniaceae and its consequences on the evolution, physiology, and stress resilience of the coral holobiont have yet to be fully elucidated. The functioning of the holobiont as a whole is largely unknown, although bacteria and viruses are presumed to play... |
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| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00593/70500/68650.pdf |
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| Alberti, Adriana; Poulain, Julie; Engelen, Stefan; Labadie, Karine; Romac, Sarah; Ferrera, Isabel; Albini, Guillaume; Aury, Jean-marc; Belser, Caroline; Bertrand, Alexis; Cruaud, Corinne; Da Silva, Corinne; Dossat, Carole; Gavory, Frederick; Gas, Shahinaz; Guy, Julie; Haquelle, Maud; Jacoby, E'Krame; Jaillon, Olivier; Lemainque, Arnaud; Pelletier, Eric; Samson, Gaelle; Wessner, Mark; Acinas, Silvia G.; Royo-llonch, Marta; Cornejo-castillo, Francisco M.; Logares, Ramiro; Fernandez-gomez, Beatriz; Bowler, Chris; Cochrane, Guy; Amid, Clara; Ten Hoopen, Petra; De Vargas, Colomban; Grimsley, Nigel; Desgranges, Elodie; Kandels-lewis, Stefanie; Ogata, Hiroyuki; Poulton, Nicole; Sieracki, Michael E.; Stepanauskas, Ramunas; Sullivan, Matthew B.; Brum, Jennifer R.; Duhaime, Melissa B.; Poulos, Bonnie T.; Hurwitz, Bonnie L.; Pesant, Stephane; Karsenti, Eric; Wincker, Patrick; Bork, Peer; Boss, Emmanuel; Follows, Michael; Gorsky, Gabriel; Hingamp, Pascal; Iudicone, Daniele; Karp-boss, Lee; Not, Fabrice; Raes, Jeroen; Sardet, Christian; Speich, Sabrina; Stemmann, Lars; Sunagawa, Shinichi; Bazire, Pascal; Beluche, Odette; Besnard-gonnet, Marielle; Bordelais, Isabelle; Boutard, Magali; Dubois, Maria; Dumont, Corinne; Ettedgui, Evelyne; Fernandez, Patricia; Garcia, Esperance; Aiach, Nathalie Giordanenco; Guerin, Thomas; Hamon, Chadia; Brun, Elodie; Lebled, Sandrine; Lenoble, Patricia; Louesse, Claudine; Mahieu, Eric; Mairey, Barbara; Martins, Nathalie; Megret, Catherine; Milani, Claire; Muanga, Jacqueline; Orvain, Celine; Payen, Emilie; Perroud, Peggy; Petit, Emmanuelle; Robert, Dominique; Ronsin, Murielle; Vacherie, Benoit. |
| A unique collection of oceanic samples was gathered by the Tara Oceans expeditions (2009-2013), targeting plankton organisms ranging from viruses to metazoans, and providing rich environmental context measurements. Thanks to recent advances in the field of genomics, extensive sequencing has been performed for a deep genomic analysis of this huge collection of samples. A strategy based on different approaches, such as metabarcoding, metagenomics, single-cell genomics and metatranscriptomics, has been chosen for analysis of size-fractionated plankton communities. Here, we provide detailed procedures applied for genomic data generation, from nucleic acids extraction to sequence production, and we describe registries of genomics datasets available at the... |
| Tipo: Text |
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| Ano: 2017 |
URL: https://archimer.ifremer.fr/doc/00600/71256/69634.pdf |
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| Registros recuperados: 13 | |
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